T7816

Sigma

 

Monoclonal Anti-β-Tubulin I antibody produced in mouse

clone SAP.4G5, ascites fluid

MDL number:MFCD02095890

Description

Immunogensynthetic peptide corresponding to the C-terminal sequence of β-tubulin isotype I, conjugated to BSA.
SpecificityRecognizes an epitope located in the C-terminal sequence of β-tubulin isotype I. No reactivity with other tubulin isotypes is observed.

Properties

antibody formascites fluid
cloneSAP.4G5 (monoclonal)
mol wtantigen mol wt ~55 kDa
species reactivitycanine, hamster, human, Xenopus, rat, bovine, mouse, chicken
application(s)immunoblotting: 1:20,000 using cultured chicken fibroblasts
 immunocytochemistry: suitable
 immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
 indirect ELISA: suitable
 microarray: suitable
isotypeIgG1
Gene Informationhuman ... TUBB(203068)
 mouse ... Tubb4(22153)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

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References

referenceRoach, M.C., et al. Cell Motil. Cytoskeleton 39, 273, (1998) Abstract
 Besserglick, H., et al., Mutant p53 protects cells from 12-O-Tetradecanoylphorbol-13-acetate-induced death by attenuating activating transcription factor 3 induction. Cancer Res. 66, 10750-10759, (2006) Abstract
 Aziz, A., et al., Development of Macrophages with Altered Actin Organization in the Absence of MafB Mol. Cell. Biol. 26, 6808-6818, (2006) Abstract
 Banerjee, A., et al., Direct involvement of the isotype-specific C-terminus of β-tubulin in ciliary beating J. Cell Sci. 118, 4333-4341, (2005) Abstract